Takahiro Kotani, Yuki Miyata, Yosuke Tsuboyama, Yuki Fujii, Takaharu Okajima*, Local Intracellular stiffening of ascidian embryo in cleavage developmental stage observed by atomic force microscopy, Japanese Journal of Applied Physics in revision
Yuki Miyata, Feng-Yueh Chan, Takayuki Uchihashi, Takaharu Okajima*, Compressed sensing reconstruction of cell mechanical images obtained from atomic force microscopy, Japanese Journal of Applied Physics in revision
Toki Kaneshima, Masaki Ogawa, Takayoshi Yamamoto, Yosuke Tsuboyama, Yuki Miyata, Takahiro Kotani, Takaharu Okajima, Tatsuo Michiue*, Enhancement of neural crest formation by mechanical force in Xenopus development, International Journal of Developmental Biology (2024)
K. Shigemura, K. Kuribayashi-Shigetomi, R. Tanaka, H. Yamasaki, and T. Okajima*, Mechanical properties of epithelial cells in domes investigated using atomic force microscopy, Frontiers in Cell and Developmental Biology 11, 1245296 (2023). [paper]
T. Okajima*, K. Kuribayashi-Shigetomi, ,Atomic force microscopy for investigating cell and tissue mechanics as heterogeneous and hierarchical materials, Journal of Biomechanical Science and Engineering (2023). [paper]
H. Tsuru, C. Yoshihara, H. Suginobe, M. Matsumoto, Y. Ishii, J. Narita , R. Ishii, R. Wang, A. Ueyama, K. Ueda, M. Hirose, K. Hashimoto, H. Nagano, R. Tanaka, T. Okajima, K. Ozono, H. Ishida* ,Pathogenic Roles of Cardiac Fibroblasts in Pediatric Dilated Cardiomyopathy, Journal of the American Heart Association 12,e029676 (2023). [paper]
M. Matsumoto#, H. Tsuru#, H. Suginobe#, J. Narita, R. Ishii, M. Hirose, K. Hashimoto, R. Wang, C. Yoshihara, A. Ueyama, R. Tanaka, K. Ozono, T. Okajima*, H. Ishida* (# equal contribution), Atomic force microscopy identifies the alteration of rheological properties of the cardiac fibroblasts in idiopathic restrictive cardiomyopathy,PLoS ONE 17, e0275296 (2022). [paper]
Y. Miyata, T. Kotani, Y. Tsuboyama, T. Matsuo, T. Okajima, Atomic force microscopy reveals a relationship between rheological properties of cells and cell fates in the early developmental stages(31st International Colloquium on Scanning Probe Microscopy、2023.12.7-8、Tokyo)
T. Kotani, Y. Miyata, Y. Tsuboyama, T. Matsuo, M. Yokobori, Y. Fujii, T. Okajima、Spatiotemporal dynamics of single-cell rheology in developing embryos observed by atomic force microscopy.(31st International Colloquium on Scanning Probe Microscopy、2023.12.7-8、Tokyo)
Y. Miyata, T. Kotani, Y. Tsuboyama, T. Matsuo, T. Okajima, Rheological properties of developing embryo in gastrula-stage depend on the cell fates: an atomic force microscopy study(第61回日本生物物理学会年会、2023.11.14-16、名古屋)
H.Yamasaki, K.Kuribayashi-Shigetomi, M.Kojima, T.Okajima、Rheological properties of Submucosal and subperitoneal fibroblasts measured by atomic force microscopy(第61回日本生物物理学会年会、2023.11.14-16、名古屋)
T. Kotani, Y. Miyata, Y. Tsuboyama, T. Matsuo, M. Yokobori, Y. Fujii, T. Okajima、Universality of single-cell rheology during cell division in developing embryo observed by atomic force microscopy.(第61回日本生物物理学会年会、2023.11.14-16、名古屋)
Y. Miyata, T. Kotani, Y. Tsuboyama, T. Matsuo, Y. Fujii, T.Okajima, Rheological properties of single cells in developing embryo depend on the cell fates: an atomic force microscopy study (第56回日本発生生物学会、2023年7月22日ー25日、仙台)
T. Kotani, Y. Miyata, Y.Tsuboyama, M. Yokobori, T. Matsuo,Y. Fujii, T. Okajima, Quantifying rheological properties of embryonic cells in cleavage stage by atomic force microscopy(第56回日本発生生物学会、2023年7月22日ー25日、仙台)
K. Shigemura, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka and T. Okajima, Quantifying tension and elastic modulus of epithelial cells in 3D dome by atomic force microscopy (The 22nd International Vacuum Congress (IVC-22), 2022.9.11-16, Sapporo)
M. Nakada, Y. Tsuboyama, and T. Okajima, Simulating stress relaxation curves of soft materials obtained in atomic force microscopy experiments(The 22nd International Vacuum Congress (IVC-22), 2022.9.11-16, Sapporo)
T. Okajima、Embryo mechanics probed by atomic force microscopy(Complex mechanics of the cellular system(細胞システムの複雑なメカニクス), 第61回日本生物物理学会年会、2023.11.14-16、名古屋)
T. Okajima, Mapping mechanical properties of single cells during embryogenesis using atomic force microscopy(AFM BioMED conference, 2022.8.29-9.2, Okazaki)
Y. Fujii, W.C. Koizumi, T. Imai, M. Yokobori, T. Matsuo, K. Oka, K. Hotta*, T. Okajima*, Spatiotemporal dynamics of single cell stiffness in the early developing ascidian chordate embryo, Communications Biology 4, 341 (2021). [paper]
S. Katsuragi#, N. Tatsumi#, M. Matsumoto#, J. Narita, R. Ishii, H. Suginobe, H. Tsuru, R. Wang, S. Kogaki, R. Tanaka, K. Ozono, T. Okajima*, and H. Ishida*(# equal contribution),Pharmacological alteration of cellular mechanical properties in pulmonary arterial smooth muscle cells of idiopathic pulmonary arterial hypertension, Cardiology Research (2021) accepted
学会発表
Y. Tsuboyama*, M. Yokobori, T. Matsubo, Y. Fujii and T. Okajima, Stress relaxation behavior of developing embryo measured by atomic force microscopy (29th International Colloquium on Scanning Probe Microscopy (ICSPM29), Dec. 9-10, 2021, Online).
K. Shigemura*, K.K. Shigetomi, A. Subagyo, K. Sueoka and T. Okajima, Quantifying mechanical property of epithelial cell dome by atomic force microscopy (29th International Colloquium on Scanning Probe Microscopy (ICSPM29), Dec. 9-10, 2021, Online).
M. Nakada* and T. Okajima, A numerical simulation of stress relaxation atomic force microscopy (29th International Colloquium on Scanning Probe Microscopy (ICSPM29), Dec. 9-10, 2021, Online).
Mataka Nagano, Miyu Ogawa, Yuki Fujii, Takaharu Okajima, Observations of structural change in epithelial dome under constant electric field (第59回日本生物物理学会年会、2021.11.25-27, online)
T. Okajima, Nanomechanics of single cells and tissues revealed by atomic force microscopy (Pacifichem 2021, Experimental and Computational Analysis of the Nano-Bio Interface for Sustainable Nanotechnology, Dec.16-21, 2021, Online)
T. Okajima, Measuring mechanical properties of developing embryo with AFM (MRS Fall meeting 2021, Frontiers in Scanning Probe Microscopy—Beyond Imaging of Soft Materials, 2021.12.6-8, Boston/Online Hybrid)
Y. M. Efremov*, T. Okajima, A. Raman, Measuring viscoelasticity of soft biological samples using atomic force microscopy, Soft Matter 16, 64-81(2020).[paper]
R. Tanaka, M. Sawano, Y. Fujii, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, T. Okajima*, Relationship between rheological properties and actin filaments of single cells investigated by atomic force microscopy, Japanese Journal of Applied Physics (2020).[paper]
K. Toyota#, R. Tanaka#, T. Okajima* (# equal contribution), Stiffness of brewers’ yeast under ethanol stress investigated by atomic force microscopy, Japanese Journal of Applied Physics 59, SN1005(2020).[paper]
M.Matsumoto*, Intracellular structures and mechanical properties of cardiac fibroblasts from pediatric patients with restrictive cardiomyopathy (Poster Award, 28th International Colloquium on Scanning Probe Microscopy (ICSPM28), Dec. 10-11, online)(co-authors: H.Suginobe, H.Tsuru, J.Narita, S.Kogaki, H.Ishida, T.Okajima)
招待講演・依頼講演
T. Okajima, Nanomechanics of cell and tissue probed by atomic force microscopy, 6th International Conference on Electronic Materials and Nanotechnology for Green Environment (ENGE 2020) (2020. 11.1-4, Korea_online)
Y. Fujii, Y. Ochi, M. Tsuchiya, M. Kajita, Y. Fujita, Y. Ishimoto, T. Okajima*, Spontaneous spatial correlation of elastic modulus in jammed epithelial monolayers observed by AFM, Biophysical Journal 116, 1152-1158 (2019).[paper]
H. Nishino*, M. Domae, T. Takanashi, T. Okajima, Cricket tympanal organ revisited: morphology, development, and possible functions of the adult-specific chitin core beneath the anterior tympanal membrane, Cell and Tissue Research (2019).[paper]
Y. Fujii, T. Okajima* , Calibrating the Young’s modulus of soft materials with surface tilt angle measured by atomic force microscopy, AIP Advances 9, 015028 (2019).[paper]
M. Tanaka#, Y. Fujii#, K. Hirano, T. Higaki, A. Nagasaki, R. Ishikawa, T. Okajima, K. Katoh* (# equal contribution), Fascin in lamellipodia contributes to cell elasticity by controlling the orientation of filamentous actin, Genes to Cells (2019). [paper]
K.Toyota*, R.Tanaka, T.Okajima, Elasticity of Brewery Yeast under Ethanol Stress Investigated by Atomic Force Microscopy (27th International Colloquium on Scanning Probe Microscopy (ICSPM27), Dec. 5-7, 2019, Shuzenji).
R.Tanaka*, M.Sawano, K.Kuribayashi-Shigetomi, T.Okajima, Relationship between Rheological Properties and Actin Density in Single Cells Investigated by AFM (27th International Colloquium on Scanning Probe Microscopy (ICSPM27), Dec. 5-7, 2019, Shuzenji).
M.Matsumoto*, H.Suginobe, S.Katsuragi, H.Tsuru, J.Narita, S.Kogaki, H.Ishida, T.Okajima、Mechanics of cardiac fibroblasts from child patients with restrictive cardiomyopathy investigated by atomic force microscopy(The 3rd JCS Council Forum on Basic Cardiac Vascular Research, 第3回日本循環器学会基礎研究フォーラム、2019年9月6日-8日、東京)
Mizuki Matsumoto*、Mechanics of cardiac fibroblasts from child patients with restrictive cardiomyopathy investigated by atomic force microscopy(Poster Award, The 3rd JCS Council Forum on Basic Cardiac Vascular Research, 第3回日本循環器学会基礎研究フォーラム)(共著者:H. Suginobe, S. Katsuragi, H. Tsuru, J. Narita, S. Kogaki, H. Ishida, T. Okajima)
Y. Miyatake*, K. Kuribayashi-Shigetomi*, Y. Ohta, S. Ikeshita, A. Subagyo, K. Sueoka, A. Kakugo, M. Amano, T. Takahashi, T. Okajima, M. Kasahara, Visualising the dynamics of live pancreatic microtumours self-organised through cell-in-cell invasion, Scientific Reports 8, 14054 (2018). [paper]
A. Tanaka*, Y. Fujii, N. Kasai, T. Okajima, H. Nakashima, Regulation of Neuritogenesis in Hippocampal Neurons using Stiffness of Extracellular Microenvironment. PLOS ONE 13, e0191928(2018) [paper]
Q. He, T. Okajima, H. Onoe, A. Subagyo, K. Sueoka, K. Kuribayashi-Shigetomi*, Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures, Scientific Reports 8, 4556 (2018) [paper]
Y. Fujii*, T. Imai, W. Koizumi, K. Hotta, K. Oka, T. Okajima, Spatiotemporal Change in Cell Stiffness during Early Embryogenesis Investigated by Atomic Force Microscopy (Biophysical Society 62nd Annual Meeting, San Francisco,Feb 17-21, USA)
T. Okajima*, R. Tanaka, M. Nagase, K. Ogura, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, Single Cancer Cell Diagnosis Based on Power-Law Rheology by AFM (ACSIN-14&ICSPM26, 2018年10月21-25日、仙台)
Y. Fujii*, T. Okajima, Mapping cell stiffness of living embryo during the developmental process by atomic force microscopy (ACSIN-14&ICSPM26, 2018年10月21-25日、仙台)
P.G. Cai, R. Takahashi, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, J. M. Maloney, K. J. Van Vliet, T. Okajima*, Temporal Variation in Single-Cell Power-Law Rheology Spans the Ensemble Variation of Cell Population, Biophysical Journal 113, 671-678 (2017) . [paper] (This paper was highlighted in BJ.)
S.‑O. Kim, J. Kim, T. Okajima and N.‑J. Cho*, Mechanical properties of paraformaldehyde‑treated individual cells investigated by atomic force microscopy and scanning ion conductance microscopy. Nano Convergence 4, 5 (2017).[paper]
Y.Fujii*, T. Imai, W. Koizumi, K. Hotta, K. Oka, and T.Okajima, Spatiotemporal change in cell stiffness during early embryogenesis investigated by atomic force microscopy (62nd Annual Meeting Biophysical Society, 2018.2.17-21, California).
Y.Fujii* and T.Okajima, Quantifying elastic modulus of soft samples with tilting angle by atomic force microscopy (25th International Colloquium on Scanning Probe Microscopy (ICSPM25), Dec. 7-9, 2017, Atagawa).
M.Nagase*, R.Tanaka, K.Kurubayashi-Shigetomi, A.Subagyo, K.Sueoka, T.Okajima, Mechanical Diagnosis of Micropatterned Cells by AFM(25th International Colloquium on Scanning Probe Microscopy (ICSPM25), Dec. 7-9, 2017, Atagawa).
R. Takahashi, T. Okajima*, Comparison between power-law rheological parameters of living cells in frequency and time domains measured by atomic force microscopy. Japanese Journal of Applied Physics 55, 08NB22 (2016).[paper]]
T. Okajima,Nanorheology of Living Cells (Chapter 13, The World of Nano-Biomechanics, A. Ikai, Elsevier) (2016)
M. Sawano*, K. Aoki, R. Tanaka, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, N.J. Cho, T. Okajima, Diagnosing single cell diseases by SPM (24th International Colloquium on Scanning Probe Microscopy (ICSPM24), Hawaii, Dec 14-16, 2016)
R. Tanaka*, R. Aritomo, T. Shioi, T. Okajima, The number distribution of elastic modulus of brewery yeast investigated by atomic force microscopy (2016 ASCB Annual Meeting, San Francisco, Dec 3-7, 2016)
M. Sawano, R. Tanaka, R. Takahashi, K. Kurubayashi-Shigetomi, A. Subagyo, K. Sueoka, T.Okajima*, Mapping cell-to-cell variations in power-law rheology investigated by multifrequency force modulation atomic force microscopy (2016 ASCB Annual Meeting, San Francisco, Dec 3-7, 2016)
Y. Fujii*, T. Okajima, Spatial mechanical heterogeneity in epithelial cell sheet : an atomic force microscopy study (Mechanobiology of Disease, Singapore, Sep. 27-30, 2016)
Takaharu Okajima, Mapping elastic modulus of single cells and tissue in stable state and morphogenesis by atomic force microscopy, 4th Kanazawa Bio-AFM Workshop,Oct 3 – 6, 2016, Kanazawa, Japan
R. Takahashi, T. Okajima*, Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy, Applied Physics Letters 107, 173702 (2015).[paper]
A. Tanaka*, R. Tanaka, N. Kasai, S. Tsukada, T. Okajima, K. Sumitomo, Time-lapse imaging of morphological changes in a single neuron during the early stages of apoptosis using scanning ion conductance microscopy, Journal of Structural Biology 191, 32–38 (2015). [paper]
P.G. Cai, T. Okajima*, Precision of cell-to-cell variation in power-law rheology characterized by atomic force microscopy, Japanese Journal of Applied Physics 54, 037001(2015)[paper]
T. Okajima, Atomic Force Microscopy: Imaging and Rheology of Living Cells (Nano/Micro Science and Technology in Biorheology: Principles, Methods, and Applications Chapter 15 (387-414), Springer, 2015)[book]
K. Kuribayashi-Shigetomi, R. Takahashi, A. Subagyo, K. Sueoka, T. Okajima, High-throughput measurements of single cell rheology by atomic force microscopy (Hyper Bio Assembler for 3D Cellular Systems, Chapter 4 (57-67), Springer, 2015)[book]
Y. Fujii*, W. Koizumi, K. Hotta, K. Oka, T. Okajima, Quantifying local elastic modulus of living embryo by atomic force microscopy(23rd International Colloquium on Scanning Probe Microscopy (ICSPM23), Dec. 10-12, 2015, Niseko)
R. Takahashi*, T. Okajima, Multi-frequency force modulation atomic force microscopy:Simultaneous measurements of living cells in frequency and time domains (23rd International Colloquium on Scanning Probe Microscopy (ICSPM23), Dec. 10-12, 2015, Niseko)
Ryosuke Tanaka*, Takaharu Okajima, Mechanical Measurements of in vitro Tissue by Atomic Force Microscopy (26th 2015 International Symposium on Micro-NanoMechatronics and Human Science, Nov.23-25,2015 Nagoya)
Yuki Fujii*, Wataru Koizumi, Koji Hotta and Takaharu Okajima, Spatial-Temporal Oscillation in Elastic Modulus of Embryo during the Early Development, International Symposium on Fluctuation and Structure Out of Equilibrium 2015 (SFS2015) (H25.8.21-23, Kyoto)
M. Kajita, K. Sugimura, A. Ohoka, J. Burden, H. Suganuma, M. Ikegawa, T. Shimada, T. Kitamura, M. Shindoh, S. Ishikawa, S. Yamamoto, S. Saitoh, Y. Yako, R. Takahashi, T. Okajima, J. Kikuta, Y. Maijima, M. Ishii, M. Tada, and Y. Fujita*, Filamin acts as a key regulator in epithelial defence against transformed cells, Nature Communications 5, 4428(2014)[paper]
R.Takahashi, S. Ichikawa, A. Subagyo, K. Sueoka, and T. Okajima*, Atomic force microscopy measurements of mechanical properties of single cells patterned by microcontact printing, Advanced Robotics 28, 449-455(2014) [paper]
岡嶋孝治,細胞の物理学:SPMによる定量計測、表面科学 35, 544-544(2014)
岡嶋孝治,原子間力顕微鏡による細胞力学の定量計測(解説)、検査技術 4,2-6(2014)
学会発表
P.G. Cai*, T. Okajima, Origin of cell-to-cell mechanical variation investigated by atomic force microscopy (22nd International Colloquium on Scanning Probe Microscopy (ICSPM22), Dec 11-13,2014 Atagawa)
R. Tanaka*, J. Kikkawa, Y. Fujii, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, T. Okajima, Atomic force microscopy for mapping mechanical property of whole cell assembly (25th 2014 International Symposium on Micro-NanoMechatronics and Human Science, Nov 9-12, Nagoya)
R. Takahashi*, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, T. Okajima, Quantitative rheological measurements of confluent cell using atomic force microscopy (25th 2014 International Symposium on Micro-NanoMechatronics and Human Science, Nov 9-12, Nagoya)
P.G. Cai*, R. Takahashi, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, T. Okajima, Temporal and Spatial Variations of Single Cell Rheology Investigated by Atomic Force Microscopy (25th 2014 International Symposium on Micro-NanoMechatronics and Human Science, Nov 9-12, Nagoya)
P.G. Cai, Y. Mizutani, M. Tsuchiya, J. M. Maloney, B. Fabry, K. J. Van Vliet, T. Okajima*, Quantifying Cell-to-Cell Variation in Power-Law Rheology, Biophysical Journal 105 (2013) 1093-1102. [paper]
Y. Mizutani, M.-H. Choi, S.-J. Cho, T. Okajima*, Nanoscale fluctuations on epithelial cell surfaces investigated by scanning ion conductance microscopy, Applied Physics Letters 102 (2013) 173703 (4pages).[paper]
Ryosuke Takahashi*, Kaori Kuribayashi-Shigetomi, Masahiro Tsuchiya, Agus Subagyo, Kazuhisa Sueoka, Takaharu Okajima, High-throughput Measurements of Cell Mechanics Using Atomic Force Microscopy with Micro-patterned Substrates, 24th 2013 International Symposium on Micro-NanoMechatronics and Human Science(From Micro & Nano Scale Systems to Robotics & Mechatronics Systems)(2013. 11.11-13, Nagoya)
Yuki Ochi*, Masahiro Tsuchiya, Yuki Saito, Takaharu Okajima, Development of an atomic force microscope for measuring mechanical properties of cell population, 24th 2013 International Symposium on Micro-NanoMechatronics and Human Science(From Micro & Nano Scale Systems to Robotics & Mechatronics Systems)(2013. 11.11-13, Nagoya)
Z. Ishikura*, K. Kuribayashi-Shigetomi, Y. Mizutani, Y. Fujii, M.-H. Choi, S.-J. Cho, T. Okajima, Spatial dependence of membrane fluctuations of cells on micro-patterned substrates measured by scanning ion conductance microscopy, 12th International Conference on Atomically Controlled Surfaces, Interfaces and Nanostructures (ACSIN-12)/21st International Colloquium on Scanning Probe Microscopy(ICSPM21) (2013. 11.4-8, Tsukuba)
Y. Mizutani, Z. Ishikura, M.-H. Choi, S.-J. Cho, T. Okajima*, Nanoscale cell membrane fluctuations measured by scanning ion conductance microscopy, (Biophysical Society 57th Annual Meeting, 2013.2.1-7, Philadelphia)
Takaharu Okajima*,Quantifying spatial and temporal variation in cell mechanics by scanning probe microscopy (Invited), 12th International Conference on Atomically Controlled Surfaces, Interfaces and Nanostructures (ACSIN-12)/21st International Colloquium on Scanning Probe Microscopy(ICSPM21) (2013. 11.4-8, Tsukuba)
Y. Mizutani, Z. Ishikura, K. Shigetomi-Kuribayashi, M.H. Choi, S.J. Cho, T. Okajima*, Apical cell shape fluctuations quantified by scanning ion conductance microscopy (Invited), International Symposium on Morphological Sciences (XXIII ISMS 2013) (2013.9.10-13, Niigata)
2012年
論文・著書
T. Okajima*, Atomic Force Microscopy for the Examination of Single Cell Rheology (Review), Current Pharmaceutical Biotechnology, 13(2012)2623-2631.
Y. Mizutani, K. Kawahara, T. Okajima*, Effect of Isoproterenol on Local Contractile Behaviors of Rat Cardiomyocytes Measured by Atomic Force Microscopy Current Pharmaceutical Biotechnology 13(2012)2599-2603.
Y. Mizutani, M.-H. Choi, S.-J. Cho, and T. Okajima, Quantitative Measurement of Cell Membrane Fluctuations by Scanning Ion Conductance Microscopy,(20th International Colloquium on Scanning Probe Microscopy (2012.12.17-19, Naha)
R. Mizuto, Y. Sakai, R. Takahashi, T. Okajima, Cell cycle dependence of cell mechanics investigated by traction force microscopy,(25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), 2012.11.27-30)
R. Takahashi, Y. Mizutani, K. Kawahara and T. Okajima, Power-law rheology of normal and cancer cells investigated by atomic force microscopy, Biophysical Society 56th Annual Meeting (2012.2.24-3.1, San Diego)
M.Tsuchiya, Y.Mizutani, K.Kawahara, T.Okajima, Universal rheological properties of cells in intermediate states observed in stress relaxation of cell sheets, Biophysical Society 56th Annual Meeting (2012.2.24-3.1, San Diego)
Takaharu Okajima, Exploring mechanical properties of live cells on microfabricated substrates by atomic force microscopy (Plenary), The 7th Nagoya University-UCLA International Symposium (2012.9.21-22, Sapporo)
Takaharu Okajima,Fluctuations of single cells measured by atomic force microscopy and scanning ion conductance microscopy (Invited),3rd Kanazawa Bio-AFM Workshop (2012. 11.5-8, Kanazawa)
2011年
論文・著書
A. Okada, Y. Mizutani, A. Subagyo, H. Hosoi, M. Nakamura, K. Sueoka, K. Kawahara, T. Okajima*,Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy. Appl. Phys. Lett. 99 (2011) 263703. [selected for the January 1, 2012 issue of Virtual Journal of Biological Physics Research]
Kunugi, S., Iwabuchi, S., Matsuyama, D., Okajima, T. and Kawahara*, K.Negative-feedback regulation of ATP release: ATP release from cardiomyocytes is strictly regulated during ischemia. Biochemical and Biophysical Research Communications, 416:409-415, 2011.
Niikura K*, Nambara K, Okajima T, Kamitani R, Aoki S, Matsuo Y, Ijiro K.
Artificial polymeric receptors on the cell surface promote the efficient cellular uptake of quantum dots, Org Biomol Chem. 9(2011)5787-92.
A. Miyaoka, Y. Mizutani, M. Tsuchiya, K. Kawahara, T. Okajima* Rheological Properties of Growth-Arrested Fibroblast Cells under Serum Starvation Measured by Atomic Force Microscopy. Jpn. J. Appl. Phys. 50 (2011)08LB16(4pages).
学会発表
P.G. Cai, Y. Mizutani, K. Kawahara and T. Okajima, Temporal Variation of Single Cell Rheology Investigated by Atomic Force Microscopy, 19th International Colloquium on Scanning Probe Microscopy (2011.12.19-21, Toyo)
Y. Mizutani and T. Okajima, Ion current mapping of living cells using ion conductance microscopy, 19th International Colloquium on Scanning Probe Microscopy (2011.12.19-21, Toyo)
Y. Usuki, Y. Mizutani, M. Tsuchiya and T. Okajima, Cell Cycle Dependence of Single Cell Rheology Investigated by AFM, 19th International Colloquium on Scanning Probe Microscopy (2011.12.19-21, Toyo)
R. Takahashi, Y. Mizutani, K. Kawahara and T. Okajima, Power-law rheology of normal and cancer cells investigated by atomic force microscopy, 19th International Colloquium on Scanning Probe Microscopy (2011.12.19-21, Toyo)
PingGen Cai, Yusuke Mizutani, Masahiro Tsuchiya, Koichi Kawahara and Takaharu Okajima, Spatial and Temporal Dependence of Single Cell Rheology Investigated by Atomic Force Microscopy, Biophysical Society 55th Annual Meeting (March 4-9, 2011, Baltimore)
M. Tsuchiya, Y. Mizutani, K. Kawahara and T. Okajima, Poisson’s ratio of a cell sheet determined in uniaxial stretching experiments, Biophysical Society 55th Annual Meeting (March 4-9, 2011, Baltimore)
K. Niikura*, K. Nambara, T. Okajima, Y. Matsuo and K. Ijiro, Influence of Hydrophobic Structures on the Plasma Membrane Permeability of Lipidlike Molecules Langmuir 26 (2010) 9170
S. Hiratsuka, Y. Mizutani, P.G. Cai, M. Tsuchiya, H. Tokumoto, K. Kawahara, T. Okajima*, Statistics of Single Cell Mechanics Investigated by Atomic Force Microscopy The 2010 MRS Spring Meeting Symposium U proceedings in press.
P.G. Cai, Y. Mizutani, A. Miyaoka, M. Tsuchiya, K. Kawahara and T. Okajima, Time and Space Dependent Studies in Cell Rheology by Atomic Force Microscopy, 18th International Colloquium on SPM (2010 12 9-11, Atagawa)
A. Miyaoka, Y. Mizutani, M. Tsuchiya, K. Kawahara and T. Okajima, Rheology of Single Cells under Serum Starvation Measured by Atomic Force Microscopy, 18th International Colloquium on SPM (2010 12 9-11, Atagawa)
M. Tsuchiya, Y. Mizutani, K. Kawahara and T. Okajima, Mechanics of a cell sheet investigated by uniaxial stretching method (細胞シート一軸延伸法による細胞メカニクスの研究), 第48回日本生物物理学会年会,(2010.9.20-9.22,仙台)
M. Tsuchiya, Y. Mizutani, K. Kawahara and T. Okajima, Power-law rheology of monolayer cell sheets under uniaxial stretching, 5th Pacific Rim Conference on Rheology (August 1-6, 2010,Sapporo)
M. Tsuchiya, Y. Mizutani, K. Kawahara and T. Okajima, Rheology of monolayer cell sheets with periodic structure, Biophysical Society 54th Annual Meeting (February 20-24, 2010, San Francisco)
Y. Mizutani, P. G. Cai, M. Tsuchiya, K. Kawahara and T.Okajima,Rheology of transformed cells investigated by AFM, International Scanning Probe Microscopy Conference Sapporo ’10 (2010.5.10-12,札幌)
Y. Mizutani, P. G. Cai, M. Tsuchiya, K. Kawahara, and T. Okajima,Rheology of transformed fibroblast cells investigated by atomic force microscopy, 日本生物物理学会第48回年会(2010.9.20-22,仙台)
T. Okajima, S. Hiratsuka, Y. Mizutani, P.G. Cai, M. Tsuchiya, H. Tokumoto and K. Kawahara,Statistics of Single Cell Mechanics Investigated by Atomic Force Microscopy, (2010 MRS Spring Meeting, San Francisco, April 5-9,2010)
S. Hiratsuka, Y. Mizutani, M. Tsuchiya, K. Kawahara, H. Tokumoto and T. Okajima*,The number distribution of complex shear modulus of single cells measured by atomic force microscopy,Ultramicroscopy Volume 109, Issue 8, July 2009, Pages 937-941.
R. Kamitani, K. Niikura*, T. Okajima, Y. Matsuo, K. Ijiro*, Design of cell-surface-retained polymers for artificial ligand display, Chembiochem 10(2009)230-233.
S. Hiratsuka, Y. Mizutani, A. Toda, N. Fukushima, K. Kawahara, H. Tokumoto and T. Okajima*, Power-Law Stress and Creep Relaxations of Single Cells Measured by Colloidal ProbeAtomic Force Microscopy Jpn. J. Appl. Phys. 48 (2009)08JB14-1-4
Y. Mizutani, D. Matsumaya, S. Hiratsuka, K. Kawahara, H. Tokumoto, and T. Okajima. Mechanics of Spontaneously Beating Cardiac Myocytes Investigated by AFM, The Biophysical Society 53nd Annual Meeting, CD-ROM (The Biophysical Society 53nd Annual Meeting, Boston, MA, U.S.A., February, 2009)
Y. Mizutani, M. Tsuchiya, K. Kawahara and T. Okajima, Machanics of cardiac cells under hypertrophy induced by oxidative stress measured with an atomic force microscope(原子間力顕微鏡を用いた酸化ストレスによる細胞肥大過程の力学特性評価), 第47回日本生物物理学会年会,ppS192(第47 回日本生物物理学会年会,徳島市,2008.10.30-11.01)
M. Tsuchiya, Y. Mizutani, K. Kawahara and T. Okajima, Mechanics of a cell-sheet with periodic structures(周期構造を有する細胞シートを用いた細胞力学), 第47回日本生物物理学会年会, ppS177(第47回日本生物物理学会年会, 徳島市,2008.10.30-11.01)
P.G. Cai, Y. Mizutani, A. Miyaoka, M. Tsuchiya, K. Kawahara and T. Okajima, Mechanics of Single Cells Cultured on Microarray Measured by Atomic Force Microscopy, 17th International Colloquium on Scanning Probe Microscopy (ICSPM17)(December 10-12, 2009. )
M.Tsuchiya, Y.Mizutani, K.Kawahara, T.Okajima, Rheology of monolayer cell sheet with periodic structure, Biophysical Society 54th Annual Meeting (feb. 20-24,2010, San francisco)
T. Okajima, Statistical analysis of single cell mechanics investigated by atomic force microscopy, The 4th International Congress on Stress Responses in Biology and Medicine (Symp: Novel Approaches to Study Stress Biology) (Oct. 6-9, 2009, Sapporo).
K. Shikinaka, H. Kwon, A. Kakugo, H. Furukawa, Y. Osada, J.P. Gong*, Y. Aoyama, H. Nishioka, H. Jinnai, T. Okajimaa, “Observation of the Three-Dimensional Structure of Actin Bundles formed with Polycations”, Biomacromolecules, 9, 537-542 (2008).
Y. Tanaka*, Y. Kawauchi, T. Kurokawa, H. Furukawa, T. Okajima, and J. P. Gong, “Localized Yielding around Crack Tips of Double-network Gels”, Macromol. Rapid Commun., 29, 1514-1520 (2008).
H. Yamamura, K. Kimura, T. Okajima, H. Tokumoto, Y. Watamabe*, “Affinity of Functional Groups for Membrane Surfaces: Implications for Physically Irreversible Fouling”, Environ. Sci. Technol. 42(2008) 5310-5315.
Y. Mizutani, M. Tsuchiya, S. Hiratsuka, K. Kawahara, H. Tokumoto and T. Okajima*, “Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy”, Jpn. J. Appl. Phys. 47(2008)6177-6180.
Y. Mizutani, M. Tsuchiya, S. Hiratsuka, K. Kawahara, H. Tokumoto, and T. Okajima., Mechanics of Living Cells at Early Stage of Adhesion Investigated with AFM. Joint Meeting of the Biophysical Society 52nd Annual Meeting & 16th International Biophysics Congress, Long beach, CA, U.S.A. (February, 2008)
Y. Mizutani, M. Tsuchiya, S. Hiratsuka, K. Kawahara, H. Tokumoto, and T. Okajima., Elasticity of Cardiac Cells on a Microarray under Oxidative Stress Measured by Atomic Force Microscopy. International Scanning Probe Microscopy Conference Seattle ’08, Seattle, WA, U.S.A. (June, 2008)
Y. Mizutani, M. Tsuchiya, S. Hiratsuka, K. Kawahara, H. Tokumoto, T. Okajima., Mechanics of Cardiac Cells on a Microarray under Oxidative Stress Investigated by Atomic Force Microscopy. International Conference on Nanoscience + Technology 2008, Keystone, Colorado, U.S.A. (July, 2008)
T. Okajima, Y. Mizutani, S. Hiratsuka, M. Tsuchiya, H. Tokumoto, and K. Kawahara, Statistics of cellular mechanics investigated by AFM. AFM BioMed conference 2008, Monterey, CA, U.S.A. (October, 2008)
Y. Mizutani, D. Matsumaya, S. Hiratsuka, K. Kawahara, H. Tokumoto, and T. Okajima, Contractile Function of Spontaneously Beating Cardiac Myocytes Measured by AFM. 16th International Colloquium on Scanning Probe Microscopy (ICSPM16), Atagawa, Japan (December , 2008)
Y. Mizutani, S. Hiratsuka, M. Tsuchiya, K. Kawahara, H. Tokumoto, and T. Okajima, Force Measurement of living Cells by Atomic Force Microscopy with a Microarray Technique (マイクロアレイを用いたAFMによる多数生細胞の力学計測),日本生物物理学会第46回年会,福岡市,12/2008
S.Hiratsuka, Y.Mizutani, M.Tuchiya, K.Kawahara, H.Tokumoto and T.Okajima,Viscoelastic Properties of Many Living Cells Investigated by Atomic Force Microscopy.(原子間力顕微鏡を用いた多数細胞粘弾性の精密測定)第46回 年会講演予稿集,vol.48,supplement1,ppS175,2008.(第46回 日本生物物理学会,福岡,2008.12.3-5.)
S.Hiratsuka, Y.Mizutani, K.Kawahara, H.Tokumoto and T.Okajima,The number distribution of complex shear modulus of living cells by AFM. 10th International Scanning Probe Microscopy Conference (ISPM),(Seatlle, USA, June 22-24, 2008.)
S.Hiratsuka, Y.Mizutani, K.Kawahara, H.Tokumoto and T.Okajima. Stress Relaxation and Creep Tests of Living Cells on Microarray Investigated by AFM. The 16th International Colloquium on Scanning Probe Microscopy (ICSPM16), (Atagawa, Japan, December 11-13, 2008.), JSAP:092231,pp94.
M. Tsuchiya,Y. Mizutani, K. Kawahara,T. Okajima Development of artificial cell crystal for studying cell-cell interactions(細胞間相互作用の測定のための生細胞配列構造体の開発)日本生物物理学会第46回年会,福岡市,12/2008
T. Okajima*, M. Tanaka, S. Tsukiyama, T. Kadowaki, S. Yamamoto, M. Shimomura and H. Tokumoto, ” Stress Relaxation Measurement of Fibroblast Cells with Atomic Force Microscope “, Jpn. J. Appl. Phys. (2007) 5552-5555
T. Okajima*, M. Tanaka, S. Tsukiyama, T. Kadowaki, S. Yamamoto, M. Shimomura and H. Tokumoto, “Stress relaxation of HepG2 cells measured by atomic force microscopy”, Nanotechnology 18 (2007) 084010
T. Okajima*, X.M. Tao, H. Azehara, H. Tokumoto, “Force Spectroscopy on Single Polymer Incorporated into Polymer Gels”, J. Nanosci. Nanotech. 7, 790?795 (2007).
H.-J. Kwon, A. Kakugo, T. Ura, T. Okajima, Y. Tanaka, H. Furukawa, Y. Osada and J.-P. Gong*, “Actin network formation by uni-directional polycation diffusion”, Langmuir 23(2007)6257-6262
K. Hiroaki, T. Takagi, T. Okajima, M. Kinjo*, “DNA microstructure based on self-assembly of 4-Sticky-End Holiday Junctions in Aqueous Solution “, J. Nanosci. Nanotech. 7(2007)726-729.